Perforin 1 antibody

Catalog No. ABIN6963873

The Mouse Monoclonal anti-Perforin 1 antibody has been validated for ELISA and ELISpot. It is suitable to detect Perforin 1 in samples from Human and Non-Human Primate. There are 5+ publications available.

Quick Overview for Perforin 1 antibody (ABIN6963873)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: ELISA, ELISpot

Clone: Pf-80-164

Product Details anti-Perforin 1 Antibody

Specificity: Native Perforin.

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with Perforin from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Native human Perforin

Isotype: IgG1

Application Details

Application Notes: For quantification of Perforin in solution e.g. cell culture supernatants using ELISA and for enumeration of Perforin producing cells using ELISpot. Pf-80/164 is recommended as coating mAb in combination with detection mAb Pf-344 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store product at 4-8°C.

Expiry Date: 18 months

References for anti-Perforin 1 antibody (ABIN6963873)

Hu, Valentin, Cai, Dayton, Rosati, Ramírez-Salazar, Kulkarni, Broderick, Sardesai, Wyatt, Earl, Moss, Mullins, Pavlakis, Felber: "DNA Vaccine-Induced Long-Lasting Cytotoxic T Cells Targeting Conserved Elements of Human Immunodeficiency Virus Gag Are Boosted Upon DNA or Recombinant Modified Vaccinia Ankara Vaccination." in: Human gene therapy, Vol. 29, Issue 9, pp. 1029-1043, (2019) (PubMed).

Parrot, Allard, Oger, Benlalam, Raingeard de la Blétière, Coutolleau, Preisser, Desfrançois, Khammari, Dréno, Labarrière, Delneste, Guardiola, Gervois: "IL-9 promotes the survival and function of human melanoma-infiltrating CD4(+) CD8(+) double-positive T cells." in: European journal of immunology, Vol. 46, Issue 7, pp. 1770-82, (2017) (PubMed).

Rout: "Enhanced Th1/Th17 Functions of CD161+ CD8+ T Cells in Mucosal Tissues of Rhesus Macaques." in: PLoS ONE, Vol. 11, Issue 6, pp. e0157407, (2017) (PubMed).

Chung, Hung, Yang, Su, Huang, Wei, Chin, Chiou, Chu, Ho, Yang, Lu, Wu, Liao, Chen: "Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis." in: Nature medicine, Vol. 14, Issue 12, pp. 1343-50, (2008) (PubMed).

Belyakov, Kuznetsov, Kelsall, Klinman, Moniuszko, Lemon, Markham, Pal, Clements, Lewis, Strober, Franchini, Berzofsky: "Impact of vaccine-induced mucosal high-avidity CD8+ CTLs in delay of AIDS viral dissemination from mucosa." in: Blood, Vol. 107, Issue 8, pp. 3258-64, (2006) (PubMed).

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Gene ID: 5551

Pathways: Apoptosis, Caspase Cascade in Apoptosis