IL-5 antibody

Catalog No. ABIN6963894

The Rat Monoclonal anti-IL-5 antibody has been validated for ELISA and ELISpot. It is suitable to detect IL-5 in samples from Human and Non-Human Primate. There are 5+ publications available.

Quick Overview for IL-5 antibody (ABIN6963894)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: ELISA, ELISpot

Clone: TRFK5

Product Details anti-IL-5 Antibody

Specificity: Native and recombinant IL-5

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-5 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-5 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse IL-5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963823, ABIN7448276, ABIN6963894, ABIN7489155, ABIN6964015 and ABIN6964027 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb ABIN6963895 and ABIN7489156 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-5 antibody (ABIN6963894)

Zhenjiang, Rao, Luo, Valentini, von Landenberg, Meng, Sinclair, Hoffmann, Karbach, Altmannsberger, Jäger, Peredo, Dodoo, Maeurer: "Cytokine Networks and Survivin Peptide-Specific Cellular Immune Responses Predict Improved Survival in Patients With Glioblastoma Multiforme." in: EBioMedicine, Vol. 33, pp. 49-56, (2018) (PubMed).

Palani, Elima, Ekholm, Jalkanen, Salmi: "Monocyte Stabilin-1 Suppresses the Activation of Th1 Lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 1, pp. 115-23, (2016) (PubMed).

Oseroff, Sidney, Vita, Tripple, McKinney, Southwood, Brodie, Sallusto, Grey, Alam, Broide, Greenbaum, Kolla, Peters, Sette: "T cell responses to known allergen proteins are differently polarized and account for a variable fraction of total response to allergen extracts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 4, pp. 1800-11, (2012) (PubMed).

Wang, Metcalf, Ribeiro, McClure, Kaur: "Th-1-type cytotoxic CD8+ T-lymphocyte responses to simian immunodeficiency virus (SIV) are a consistent feature of natural SIV infection in sooty mangabeys." in: Journal of virology, Vol. 80, Issue 6, pp. 2771-83, (2006) (PubMed).

Mäkitalo, Andersson, Areström, Karlén, Villinger, Ansari, Paulie, Thorstensson, Ahlborg: "ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques." in: Journal of immunological methods, Vol. 270, Issue 1, pp. 85-97, (2002) (PubMed).

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Gene ID: 3567

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour