IL-5 antibody (Biotin)

Catalog No. ABIN6963895

This anti-IL-5 antibody is a Rat Monoclonal antibody detecting IL-5 in ELISA and ELISpot. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 5+ publications.

Quick Overview for IL-5 antibody (Biotin) (ABIN6963895)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-5 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 5A10

Product Details anti-IL-5 Antibody (Biotin)

Specificity: Native and recombinant IL-5

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IL-5 from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of human IL-5 in immunoassays such as ELISpot, FluoroSpot, and ELISA. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-5

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963895 and ABIN7489156 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963823, ABIN7448276, ABIN6963894, ABIN7489155, ABIN6964015 and ABIN6964027 .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-5 antibody (Biotin) (ABIN6963895)

Zhenjiang, Rao, Luo, Valentini, von Landenberg, Meng, Sinclair, Hoffmann, Karbach, Altmannsberger, Jäger, Peredo, Dodoo, Maeurer: "Cytokine Networks and Survivin Peptide-Specific Cellular Immune Responses Predict Improved Survival in Patients With Glioblastoma Multiforme." in: EBioMedicine, Vol. 33, pp. 49-56, (2018) (PubMed).

Shastri, Stewart, Eapen, Peterson, Zaidi, Gueven, Sohal, Patel: "Opposing effects of low molecular weight heparins on the release of inflammatory cytokines from peripheral blood mononuclear cells of asthmatics." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118798, (2015) (PubMed).

Minang, Areström, Ahlborg: "ELISpot displays a better detection over ELISA of T helper (Th) 2-type cytokine-production by ex vivo-stimulated antigen-specific T cells from human peripheral blood." in: Immunological investigations, Vol. 37, Issue 4, pp. 279-91, (2008) (PubMed).

Minang, Areström, Zuber, Jönsson, Troye-Blomberg, Ahlborg: "Nickel-induced IL-10 down-regulates Th1- but not Th2-type cytokine responses to the contact allergen nickel." in: Clinical and experimental immunology, Vol. 143, Issue 3, pp. 494-502, (2006) (PubMed).

Mola, Farah, Kariuki, Nyindo, Blanton, King: "Cytokine control of the granulomatous response in Schistosoma mansoni-infected baboons: role of exposure and treatment." in: Infection and immunity, Vol. 67, Issue 12, pp. 6565-71, (1999) (PubMed).

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Gene ID: 3567

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour