IL-21 antibody

Catalog No. ABIN6964031

This Mouse Monoclonal antibody specifically detects IL-21 in Neut. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 2+ publications.

Quick Overview for IL-21 antibody (ABIN6964031)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-21 antibody is un-conjugated

Application: Neutralization (Neut)

Clone: MT216G-21-3m

Product Details anti-IL-21 Antibody

Specificity: Native and recombinant IL-21

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-21

Isotype: IgG1

Application Details

Application Notes: Neutralization of human IL-21 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: The neutralizing capacity and ND50 was determined using ELISA and ELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After 4 days the IgG-secreting B cells were quantified by ELISpot. Neutralization dose 50 (ND50): MT216G/21.3m neutralized the effect of IL-21 at a ND50value of approximately 0.05 μg/mL when HEK cells were activated with 10 ng/mL of IL-21 (A). The corresponding ND50value for human PBMC activated with 10 ng/mL IL-21 was approximately 0.2 μg/mL. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

References for anti-IL-21 antibody (ABIN6964031)

You, Zhang, Cui, Zhu, Lv, Tang, Sun: "Th9 cells promote antitumor immunity via IL-9 and IL-21 and demonstrate atypical cytokine expression in breast cancer." in: International immunopharmacology, Vol. 52, pp. 163-167, (2018) (PubMed).

Huang, Ehrnfelt, Paulie, Zuber, Ahlborg: "ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors." in: Journal of immunological methods, Vol. 417, pp. 60-66, (2015) (PubMed).

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL21

Gene ID: 59067

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response