EIF2C3 antibody (AA 1-175)

Catalog No. ABIN6971365

The Mouse Monoclonal anti-EIF2C3 antibody (Clone 4B1-F6) (ABIN6971365) specifically detects EIF2C3 in IF, ICC, IP and ChIP. The antibody is reactive with Human samples.

Quick Overview for EIF2C3 antibody (AA 1-175) (ABIN6971365)

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2C3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 4B1-F6

Product Details anti-EIF2C3 Antibody

Binding Specificity: AA 1-175

Characteristics: Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins. Ago3 antibody (mAb) (Clone 4B1-F6) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunoprecipitation, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Alternative Name: Ago3

Molecular Weight: 110 kDa

NCBI Accession: NP_079128

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway