EIF2C3 antibody (AA 1-175)
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- Target See all EIF2C3 Antibodies
- EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))
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Binding Specificity
- AA 1-175
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EIF2C3 antibody is un-conjugated
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Application
- Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
- Characteristics
- Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins. Ago3 antibody (mAb) (Clone 4B1-F6) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Immunoprecipitation, it has been shown to react with Human samples.
- Purification
- Protein G Chromatography
- Immunogen
- This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.
- Clone
- 4B1-F6
- Isotype
- IgG2a
- Top Product
- Discover our top product EIF2C3 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))
- Alternative Name
- Ago3 (EIF2C3 Products)
- Synonyms
- EIF2C3 antibody, Eif2c3 antibody, Argonaute3 antibody, ago3 antibody, eif2c3 antibody, eif2c3b antibody, si:dkey-3n22.3 antibody, AW048688 antibody, C130014L07Rik antibody, ARGONAUTE 3 antibody, T19E23.8 antibody, T19E23_8 antibody, argonaute 3, RISC catalytic component antibody, argonaute RISC catalytic component 3b antibody, argonaute RISC catalytic subunit 3 antibody, argonaute RISC catalytic component 3 antibody, ARGONAUTE 3 antibody, AGO3 antibody, Ago3 antibody, ago3b antibody
- Molecular Weight
- 110 kDa
- NCBI Accession
- NP_079128
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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