CHD1 antibody (C-Term)

Catalog No. ABIN6971529

The Rat Monoclonal anti-CHD1 antibody has been validated for WB, IF and ICC. It is suitable to detect CHD1 in samples from Human and Mouse.

Quick Overview for CHD1 antibody (C-Term) (ABIN6971529)

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CHD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 2F11

Product Details anti-CHD1 Antibody

Binding Specificity: C-Term

Characteristics: CHD1 (Chromo-ATPase/Helicase-DNA-binding protein 1 ) is a member of the CHD family of ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation. They are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency. CHD1 antibody (mAb) (Clone 2F11) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This CHD1 antibody was raised against a peptide corresponding to the C-terminus of mouse CHD1.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for CHD1

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Alternative Name: CHD1

Molecular Weight: 210 kDa

NCBI Accession: NP_031716

Pathways: Chromatin Binding