CHD4 antibody

Catalog No. ABIN6972352

The Mouse Monoclonal anti-CHD4 antibody (Clone 3F2-4) (ABIN6972352) specifically detects CHD4 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for CHD4 antibody (ABIN6972352)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3F2-4

Product Details anti-CHD4 Antibody

Characteristics: Mi-2b (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP). Mi-2 beta antibody (mAb) (Clone 3F2/4) was raised in a Mouse host. It has been validated for use in Western blot, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogen: This Mi-2 beta antibody was raised against a synthetic peptide derived from human Mi-2B.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: Mi-2 beta

Molecular Weight: 240 kDa

NCBI Accession: NP_001264