PARP1 antibody (N-Term)

Catalog No. ABIN6972484

This anti-PARP1 antibody is a Rabbit Polyclonal antibody detecting PARP1 in WB, IP and ChIP-seq. Suitable for Human.

Quick Overview for PARP1 antibody (N-Term) (ABIN6972484)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-PARP1 Antibody

Binding Specificity: N-Term

Characteristics: PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website. PARP-1 N-terminal antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP-1

Molecular Weight: 120 kDa

NCBI Accession: NP_001609

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location