SIRT2 antibody (C-Term)

Catalog No. ABIN6972719

This Rabbit Polyclonal antibody specifically detects SIRT2 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for SIRT2 antibody (C-Term) (ABIN6972719)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SIRT2 Antibody

Binding Specificity: C-Term

Characteristics: SIRT2 is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis. SIRT2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This SIRT2 antibody was raised against a synthetic peptide derived from the C-terminal region of human SIRT2.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Molecular Weight: 40 & 45 kDa

NCBI Accession: NP_036369