YAP1 antibody (C-Term)
Quick Overview for YAP1 antibody (C-Term) (ABIN6972915)
Target
See all YAP1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- C-Term
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Characteristics
- YAP1 (Yes-associated protein 1, YAP, YKI, YAP2, YAP65) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Is a critical modulator of epidermal stem cell proliferation. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. YAP1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.
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Purification
- Affinity Purified
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Immunogen
- This YAP1 antibody was raised against a peptide corresponding to the C-terminal region of human YAP1.
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Isotype
- IgG
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Buffer
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- YAP1 (Yes-Associated Protein 1 (YAP1))
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Alternative Name
- YAP1
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Molecular Weight
- 75 kDa
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NCBI Accession
- NP_001123617
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Pathways
- MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Target
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