DPF3 antibody (AA 1-100) (AbBy Fluor® 350)

Catalog No. ABIN6976496

The Rabbit Polyclonal anti-DPF3 antibody is suitable to detect DPF3 in samples from Human. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for DPF3 antibody (AA 1-100) (AbBy Fluor® 350) (ABIN6976496)

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF3 antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DPF3 Antibody (AbBy Fluor® 350)

Binding Specificity: AA 1-100

Purpose: CERD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CERD4

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DPF3

Target: DPF3 (D4, Zinc and Double PHD Fingers, Family 3 (DPF3))

Alternative Name: CERD4

Background:

Synonyms: Zinc finger protein DPF3, DPF3, BRG1-associated factor 45C, BAF45C, Zinc finger protein cer-d4, BAF45C, CERD4

Background: Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.

Gene ID: 8110

UniProt: Q92784