CHEK2 antibody (pSer33, pSer35) (AbBy Fluor® 555)

Catalog No. ABIN6978224

The Rabbit Polyclonal anti-CHEK2 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect CHEK2 in samples from Human.

Quick Overview for CHEK2 antibody (pSer33, pSer35) (AbBy Fluor® 555) (ABIN6978224)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CHEK2 Antibody (AbBy Fluor® 555)

Binding Specificity: pSer33, pSer35

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CHK2 isoform c around the phosphorylation site of Ser33 + Ser35

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2 (

Background:

Synonyms: Chk2 (phospho S33 + S35), p-Chk2 (phospho S33 + S35), bA444G7, CHK2 checkpoint homolog, CHK2_HUMAN, Serine/threonine-protein kinase Chk2, CDS 1, CDS1, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, CHK2 checkpoint homolog (S. pombe), CHK2 checkpoint homolog, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2.

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Gene ID: 11200

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle