Splicing Factor 1 antibody (pSer82) (AbBy Fluor® 555)

Catalog No. ABIN6978425

The Rabbit Polyclonal anti-Splicing Factor 1 antibody is suitable to detect Splicing Factor 1 in samples from Human. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for Splicing Factor 1 antibody (pSer82) (AbBy Fluor® 555) (ABIN6978425)

Target: Splicing Factor 1 (SF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Splicing Factor 1 Antibody (AbBy Fluor® 555)

Binding Specificity: pSer82

Purpose: phospho-SF1 (Ser82) Polyclonal Antibody, AbBy Fluor-555 Conjugated

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human splicing factor 1 around the phosphorylation site of Ser82

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: Splicing factor 1

Background:

Synonyms: splicing factor 1 (phospho S82), p-splicing factor 1 (phospho S82), BBP, D11S636, Mammalian branch point-binding protein mBBP, OTTHUMP00000069749, OTTHUMP00000069750, OTTHUMP00000069752, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162.

Background: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Gene ID: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location