IRS1 antibody (pTyr632) (AbBy Fluor® 555)

Catalog No. ABIN6978891

This anti-IRS1 antibody is a Rabbit Polyclonal antibody detecting IRS1 in FACS. Suitable for Human, Mouse, Rat, Pig, Rabbit, Dog, Cow and Sheep.

Quick Overview for IRS1 antibody (pTyr632) (AbBy Fluor® 555) (ABIN6978891)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is conjugated to AbBy Fluor® 555

Application: Flow Cytometry (FACS)

Product Details anti-IRS1 Antibody (AbBy Fluor® 555)

Binding Specificity: pTyr632

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Tyr632

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1

Background:

Synonyms: IRS1 (phospho Tyr632), IRS1 (phospho Y632), p-IRS1 (phospho Y632), HIRS 1, HIRS1, Insulin Receptor Substrate 1, IRS 1, IRS-1, IRS1, IRS1_HUMAN, OTTHUMP00000164234.

Background: Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process