TERT antibody (AA 521-620) (AbBy Fluor® 594)

Catalog No. ABIN6978948

This anti-TERT antibody is a Rabbit Polyclonal antibody detecting TERT in WB, FACS and IF (cc). Suitable for Human and Mouse.

Quick Overview for TERT antibody (AA 521-620) (AbBy Fluor® 594) (ABIN6978948)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TERT antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-TERT Antibody (AbBy Fluor® 594)

Binding Specificity: AA 521-620

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TERT

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: TERT

Background:

Synonyms: EST2, hEST2, TCS1, Telomerase associated protein 2, Telomerase Catalytic Subunit, Telomere Reverse Transcriptase, Telomerase reverse transcriptase, TERT, TP2, TRT, TERT_HUMAN, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2.??

Background: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified, the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008].

Gene ID: 7015

UniProt: O14746

Pathways: Telomere Maintenance