CBX4 antibody (AA 401-500) (AbBy Fluor® 647)

Catalog No. ABIN6980050

This Rabbit Polyclonal antibody specifically detects CBX4 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for CBX4 antibody (AA 401-500) (AbBy Fluor® 647) (ABIN6980050)

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CBX4 Antibody (AbBy Fluor® 647)

Binding Specificity: AA 401-500

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CBX4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4

Background:

Synonyms: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, hPc2, CBX4

Background: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Gene ID: 8535

UniProt: O00257