Cytokeratin 5 antibody (AA 331-430) (AbBy Fluor® 750)

Catalog No. ABIN6981638

The Rabbit Polyclonal anti-Cytokeratin 5 antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Cytokeratin 5 in samples from Human, Mouse and Rat.

Quick Overview for Cytokeratin 5 antibody (AA 331-430) (AbBy Fluor® 750) (ABIN6981638)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 5 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cytokeratin 5 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 331-430

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK5

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background:

Synonyms: Cytokeratin 5, Keratin, type II cytoskeletal 5, CK-5, CK 5, CK5, Keratin-5, K5, 58 kDa cytokeratin, KRT5, DDD, EBS 2, EBS2, K5, Keratin 5 epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types, Keratin 5, Keratin Type II Cytoskeletal 5, Keratin5, KRT 5, KRT 5A, KRT5, Cytokeratin-5, Cytokeratin5, DDD, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, Keratin 5, Keratin, Keratin Type II Cytoskeletal 5, Keratin5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5.

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Gene ID: 3852