YWHAB/YWHAZ antibody (pSer184, pSer186) (Biotin)

Catalog No. ABIN6982600

This Rabbit Polyclonal antibody specifically detects YWHAB/YWHAZ in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for YWHAB/YWHAZ antibody (pSer184, pSer186) (Biotin) (ABIN6982600)

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB/YWHAZ antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-YWHAB/YWHAZ Antibody (Biotin)

Binding Specificity: pSer184, pSer186

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for YWHAB/YWHAZ

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Alternative Name: 14-3-3 beta + zeta

Background:

Synonyms: 14-3-3 beta + zeta (phospho S184 + S186), 14-3-3 beta + zeta (phospho S186), 14-3-3 beta + zeta (phospho Ser186), p-14-3-3 beta + zeta (Ser186) 14 3 3 protein beta, 14 3 3 protein beta/alpha, 14 3 3 protein zeta/delta, 14 3 3 zeta, HS1, KCIP1, YWHAB, YWHAZ.

Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Gene ID: 7529

UniProt: P31946