YWHAB/YWHAZ antibody (pSer184, pSer186)

Catalog No. ABIN7224962

The Rabbit Polyclonal anti-YWHAB/YWHAZ antibody is suitable to detect YWHAB/YWHAZ in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for YWHAB/YWHAZ antibody (pSer184, pSer186) (ABIN7224962)

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB/YWHAZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-YWHAB/YWHAZ Antibody

Binding Specificity: pSer184, pSer186

Purpose: Rabbit Anti-14-3-3 β/ζ (phospho Ser184/186) Polyclonal Antibody

Specificity: Phospho-14-3-3 β/ζ (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein only when phosphorylated at S184/186.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human 14-3-3 beta/zeta Phospho-Ser184/186

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for YWHAB/YWHAZ

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Alternative Name: 14-3-3 beta/zeta

Background: YWHAZ, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB, 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1YWHAZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta) product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for YWHAZ.

Molecular Weight: 28kD

Gene ID: 7534, 7529

UniProt: P63104, P31946