YAP1 antibody (AA 51-150) (Biotin)

Catalog No. ABIN6982880

The Rabbit Polyclonal anti-YAP1 antibody has been validated for WB. It is suitable to detect YAP1 in samples from Human and Mouse.

Quick Overview for YAP1 antibody (AA 51-150) (Biotin) (ABIN6982880)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-YAP1 Antibody (Biotin)

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Sheep,Pig,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human YAP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background:

Synonyms: 65 kDa Yes associated protein, YAp 1, YAP 65, YAP, YAP2, YAP65, Yes associated protein 1 65 kDa, Yes associated protein 1, Yes associated protein 2, YKI, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1_HUMAN.

Background: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction.
Isoform 2: Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Gene ID: 10413

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha