SMAD3 antibody (AA 371-425) (Biotin)

Catalog No. ABIN6982982

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for IHC (p). It is suitable to detect SMAD3 in samples from Mouse and Rat.

Quick Overview for SMAD3 antibody (AA 371-425) (Biotin) (ABIN6982982)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMAD3 Antibody (Biotin)

Binding Specificity: AA 371-425

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Smad3

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: Smad3

Background:

Synonyms: hMAD 3, hSMAD3, HSPC193, JV15 2, JV152, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD3, MADH 3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD 3, SMAD, SMAD-3, SMAD3_HUMAN.

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gene ID: 4088

UniProt: Q15796

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy