ALKBH5 antibody (AA 1-100) (Cy7)

Catalog No. ABIN6986309

The Rabbit Polyclonal anti-ALKBH5 antibody has been validated for IF (cc) and IF (p). It is suitable to detect ALKBH5 in samples from Mouse and Rat.

Quick Overview for ALKBH5 antibody (AA 1-100) (Cy7) (ABIN6986309)

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH5 antibody is conjugated to Cy7

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ALKBH5 Antibody (Cy7)

Binding Specificity: AA 1-100

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALKBH5

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALKBH5

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Alternative Name: ALKBH5

Background:

Synonyms: ABH5, AlkB, alkylation repair homolog 5 (E. coli), AlkB, alkylation repair homolog 5, Alkylated DNA repair protein alkB homolog 5, Alpha ketoglutarate dependent dioxygenase alkB homolog 5, OFOXD, OFOXD1, Oxoglutarate and iron-dependent oxygenase domain containing, Probable alpha ketoglutarate dependent dioxygenase ABH5, RNA demethylase ALKBH5.

Background: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A)RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).

Gene ID: 54890

UniProt: Q6P6C2