TAP Tag antibody (HRP)

Catalog No. ABIN6987469

Product Details

Target: TAP Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Tag

Cross-Reactivity (Details): TAP-Tag

Purification: Purified by Protein A.

Immunogen: Recombinant TAP-Tag

Clone: Mix-mA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TAP Tag

Alternative Name: TAP-Tag

Target Type: Tag

Background:

Synonyms: TAP Tag, TAPTag, Tandem affinity purification

Background: Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.