E2F3 antibody (AA 361-460) (HRP)

Catalog No. ABIN6987723

This anti-E2F3 antibody is a Rabbit Polyclonal antibody detecting E2F3 in WB, ELISA, IHC (p) and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for E2F3 antibody (AA 361-460) (HRP) (ABIN6987723)

Target: E2F3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E2F3 Antibody (HRP)

Binding Specificity: AA 361-460

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human E2F3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for E2F3

Target: E2F3

Background:

Synonyms: DKFZp686C18211, E2F 3, E2F transcription factor 3, KIAA0075, MGC104598, Transcription factor E2F 3, E2F3_HUMAN, Transcription factor E2F3, E2F-3.

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].

Gene ID: 1871

UniProt: O00716