PTEN antibody (pSer380, pThr382, pThr383) (PE)

Catalog No. ABIN6988661

The Rabbit Polyclonal anti-PTEN antibody has been validated for WB and FACS. It is suitable to detect PTEN in samples from Human, Mouse and Rat.

Quick Overview for PTEN antibody (pSer380, pThr382, pThr383) (PE) (ABIN6988661)

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTEN antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PTEN Antibody (PE)

Binding Specificity: pSer380, pThr382, pThr383

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PTEN around the phosphorylation site of Ser380/Thr382/Thr383

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Alternative Name: PTEN

Background:

Synonyms: PTEN(Phospho-Ser380/Thr382/Thr383), PTEN(phospho S380/T382/Thr383), PTEN(Phospho-Ser380/Thr382/Thr383), ITGA 2, MGC11227, MHAM, MMAC 1, MMAC1, Bannayan Zonana, BZS a, Multiple hamartoma (Cowden syndrome), Mutated in Mutiple Advanced Cancers 1, Phosphatase and Tensin Homolog, Phosphatidylinositol 345 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN, Phosphatidylinositol 345 trisphosphate 3 phosphatase, Platelet antigen BR, PTEN 1, PTEN1, Tensin homolog, TEP 1, TEP1, VLA 2 Receptor Alpha Subunit.

Background: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability.

Gene ID: 5728

UniProt: P60484

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling