RRAGA + RRAGB (AA 231-313) antibody

Catalog No. ABIN6989150

The Rabbit Polyclonal anti-RRAGA + RRAGB antibody is suitable to detect RRAGA + RRAGB in samples from Mouse and Rat. It has been validated for IF (cc), IF (p), ELISA, IHC (fro), IHC (p) and ICC.

Quick Overview for RRAGA + RRAGB (AA 231-313) antibody (ABIN6989150)

Target: RRAGA + RRAGB

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 231-313

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RRAGA and RRAGB

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: RRAGA + RRAGB

Background:

Synonyms: FIP1, RAGA, FIP-1, RRAGB, Adenovirus E3 14.7 kDa-interacting protein 1, Rag A, RAg B, RagA, RAGB, Ras related GTP binding protein A, RRAGA_HUMAN, Ras-related GTP binding A? GTP-binding protein ragB, Rag B, RagB, Ras-related GTP-binding protein B, RRAGB, RRAGB_HUMAN..

Background: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.

Gene ID: 10670

UniProt: Q7L523