SMARCC1 antibody (AA 301-400)

Catalog No. ABIN6989262

The Rabbit Polyclonal anti-SMARCC1 antibody (ABIN6989262) specifically detects SMARCC1 in ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). The antibody is reactive with Rat samples.

Quick Overview for SMARCC1 antibody (AA 301-400) (ABIN6989262)

Target: SMARCC1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily C, Member 1 (SMARCC1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMARCC1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMARCC1 Antibody

Binding Specificity: AA 301-400

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SMARCC1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMARCC1

Target: SMARCC1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily C, Member 1 (SMARCC1))

Alternative Name: SMARCC1

Background:

Synonyms: AI115498, BAF 155, BAF155, BRG 1 associated factor 155, BRG1 associated factor 155, BRG1-associated factor 155, Chromatin remodeling complex BAF155 subunit, CRACC 1, CRACC1, Mammalian chromatin remodeling complex BRG 1 associated factor 155, Mammalian chromatin remodeling complex BRG1 associated factor 155, Rsc 8, Rsc8, SMARC C1, SMARCC 1, SMARCC1, SMRC1_HUMAN, SRG 3, SRG3, SWI 3, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related matrix associated actin dependent regulator of chromatin c1, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3.

Background: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Gene ID: 601732

UniProt: Q92922

Pathways: Chromatin Binding