Axin antibody (AA 421-520)

Catalog No. ABIN6989321

The Rabbit Polyclonal anti-Axin antibody has been validated for ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It is suitable to detect Axin in samples from Mouse.

Quick Overview for Axin antibody (AA 421-520) (ABIN6989321)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Axin Antibody

Binding Specificity: AA 421-520

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Axin1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: Axin1

Background:

Synonyms: AI316800, AXIN, Axin 1, Axin-1, axin1, AXIN1_HUMAN, Axis inhibition protein 1, Axis Inhibitor 1, Fu, fused, Fused, mouse, homolog of, hAxin, Kb, Ki, kinky, knobbly, MGC132911, MGC52315, PPP1R49, Protein Fused, Protein phosphatase 1 regulatory subunit 49.

Background: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88 kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Gene ID: 8312

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling