LIG4 antibody

Catalog No. ABIN6989825

The Rabbit Polyclonal anti-LIG4 antibody has been validated for WB and IF. It is suitable to detect LIG4 in samples from Human and Mouse.

Quick Overview for LIG4 antibody (ABIN6989825)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-LIG4 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2).

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4

Background:

Synonyms: LIG4,LIG4S

Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response