SMAD4 antibody

Catalog No. ABIN6989887

This Rabbit Polyclonal antibody specifically detects SMAD4 in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for SMAD4 antibody (ABIN6989887)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-SMAD4 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-450 of human SMAD4 (NP_005350.1).

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background:

Synonyms: DPC4,JIP,MADH4,MYHRS,Smad4,SMAD4

Background: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy