Transferrin Receptor antibody

Catalog No. ABIN6989966

The Rabbit Monoclonal anti-Transferrin Receptor antibody has been validated for FACS, WB, IHC, IP and IHC (p). It is suitable to detect Transferrin Receptor in samples from Human, Mouse and Rat.

Quick Overview for Transferrin Receptor antibody (ABIN6989966)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1B6

Product Details anti-Transferrin Receptor Antibody

Purification: Purified by Protein A.

Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IP(1-2 μg)
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C

Expiry Date: 12 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: Transferrin Receptor (CD71)

Background:

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Subcellular location: Secreted, Cell membrane, Melanosome

Synonyms: CD 71 antibody, CD71 antibody, CD71 antigen antibody, IMD46 antibody, OTTHUMP00000208523 antibody, OTTHUMP00000208524 antibody, OTTHUMP00000208525 antibody, p90 antibody, sTfR antibody, T9 antibody, TFR 1 antibody, TfR antibody, TfR1 antibody, TFR1_HUMAN antibody, TFRC antibody, TR antibody, Transferrin receptor (p90 CD71) antibody, Transferrin receptor protein 1, serum form antibody, Trfr antibody

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis