LY96 antibody (C-Term)

Catalog No. ABIN6990125

The Rabbit Polyclonal anti-LY96 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect LY96 in samples from Human, Mouse and Rat.

Quick Overview for LY96 antibody (C-Term) (ABIN6990125)

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LY96 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LY96 Antibody

Binding Specificity: C-Term

Purification: MD-2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-MD-2 antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-1 μ,g/mL, IHC: 2-2.5 μ,g/mL, IF: 10-20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human and rat samples, Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MD-2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MD-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LY96

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Alternative Name: MD-2

Background: MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2, e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Molecular Weight: Predicted: 19kD

Observed: 26 kD (Post-modification: 2 N-linked glycosylations) kDa

Gene ID: 23643

NCBI Accession: NP_056179

UniProt: Q9Y6Y9

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades