XBP1 antibody (AA 2-160)

Catalog No. ABIN6990193

This Mouse Monoclonal antibody specifically detects XBP1 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for XBP1 antibody (AA 2-160) (ABIN6990193)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 3H1G4

Product Details anti-XBP1 Antibody

Binding Specificity: AA 2-160

Purification: XBP-1 Monoclonal Antibody is Protein A purified.

Immunogen: Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.

Isotype: IgG1

Application Details

Application Notes: XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 4 μ,g/mL.

Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP-1

Background: XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response