GAPDH antibody (C-Term) (Biotin)

Catalog No. ABIN6990220

This Mouse Monoclonal antibody specifically detects GAPDH in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Rabbit.

Quick Overview for GAPDH antibody (C-Term) (Biotin) (ABIN6990220)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 12D3D5

Product Details anti-GAPDH Antibody (Biotin)

Binding Specificity: C-Term

Specificity: Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.

Purification: Biotin-GAPDH antibody is Protein A purified.

Immunogen: Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

Isotype: IgG

Application Details

Application Notes: Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Biotin-GAPDH antibody is supplied in PBS containing 1 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Molecular Weight: Predicted: 37 kDa

Observed: 36 kDa

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406