DOK1 antibody (C-Term)

Catalog No. ABIN6990237

The Rabbit Polyclonal anti-DOK1 antibody (ABIN6990237) specifically detects DOK1 in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for DOK1 antibody (C-Term) (ABIN6990237)

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DOK1 Antibody

Binding Specificity: AA 410-460, C-Term

Purification: DOK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1. The immunogen is located within amino acids 410 - 460 of DOK1.

Isotype: IgG

Application Details

Application Notes: DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μ,g/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DOK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DOK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: DOK1

Background: DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.

Molecular Weight: 62 kDa

Gene ID: 7011

UniProt: Q99704