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DFFA antibody (C-Term)

The Rabbit Polyclonal anti-DFFA antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect DFFA in samples from Human.
Catalog No. ABIN6990254

Quick Overview for DFFA antibody (C-Term) (ABIN6990254)

Target

See all DFFA Antibodies
DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This DFFA antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

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    C-Term

    Purification

    DFF45 Antibody is Antibody is DEAE purified.

    Immunogen

    DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.

    Isotype

    IgG
  • Application Notes

    DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μ,g/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    DFF45 Antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

    Alternative Name

    DFF45

    Background

    DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

    Molecular Weight

    45 kDa

    Gene ID

    1676

    NCBI Accession

    NP_004392

    UniProt

    O00273

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis
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