ASK1 antibody (C-Term)

Catalog No. ABIN6990257

The Rabbit Polyclonal anti-ASK1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect ASK1 in samples from Human.

Quick Overview for ASK1 antibody (C-Term) (ABIN6990257)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ASK1 Antibody

Binding Specificity: C-Term

Purification: ASK1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. The sequence is different from that of mouse by last two amino acids. The immunogen is located within the last 50 amino acids of ASK1.

Isotype: IgG

Application Details

Application Notes: ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μ,g/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ASK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ASK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α,-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Molecular Weight: 155 kDa

Gene ID: 4217

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response