DNASE2 antibody (C-Term)

Catalog No. ABIN6990276

This anti-DNASE2 antibody is a Rabbit Polyclonal antibody detecting DNASE2 in WB, ELISA, IHC (p) and IF. Suitable for Human.

Quick Overview for DNASE2 antibody (C-Term) (ABIN6990276)

Target: DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-DNASE2 Antibody

Binding Specificity: C-Term

Purification: DNase II Antibody is affinity chromatography purified via peptide column.

Immunogen: DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II.

Isotype: IgG

Application Details

Application Notes: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μ,g/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DNase II Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DNase II antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DNASE2

Target: DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))

Alternative Name: DNase II

Background: DNase II Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.

Molecular Weight: Aprox: 40 kDa

Gene ID: 1777

UniProt: O00115