Survivin Antibody is affinity chromatography purified via peptide column.
Immunogen
Survivin antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of mouse Survivin. The immunogen is located within the last 50 amino acids of Survivin.
BIRC5
Reactivity: Human
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
mSurvivin antibody can be used for detection of survivin by Western blot at 1 μ,g/mL. An approximate 17 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Survivin Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
mSurvivin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.