SKIL antibody (N-Term)

Catalog No. ABIN6990331

This Rabbit Polyclonal antibody specifically detects SKIL in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SKIL antibody (N-Term) (ABIN6990331)

Target: SKIL (SKI-Like Oncogene (SKIL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-SKIL Antibody

Binding Specificity: N-Term

Purification: SnoN Antibody is affinity chromatography purified via peptide column.

Immunogen: SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. The immunogen is located within the first 50 amino acids of SnoN.

Isotype: IgG

Application Details

Application Notes: SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SnoN Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SnoN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SnoN

Background: SnoN Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN, SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Gene ID: 6498

NCBI Accession: NP_005405

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus