SKIL antibody (AA 590-670)

Catalog No. ABIN7217066

The Rabbit Polyclonal anti-SKIL antibody is suitable to detect SKIL in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for SKIL antibody (AA 590-670) (ABIN7217066)

Target: SKIL (SKI-Like Oncogene (SKIL))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SKIL Antibody

Binding Specificity: AA 590-670

Purpose: Rabbit Anti-SnoN Polyclonal Antibody

Specificity: SnoN Polyclonal Antibody detects endogenous levels of SnoN protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human SnoN at AA range: 590-670

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SnoN

Background: SKIL, SNO, Ski-like protein, Ski-related oncogene, Ski-related proteinSki-like protein encoded by SKIL is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.

Molecular Weight: 77kD

Gene ID: 6498

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus