PD-L1 antibody (Isoform 1)

Catalog No. ABIN6990335

The Rabbit Polyclonal anti-PD-L1 antibody is suitable to detect PD-L1 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, ELISA, IF and IHC (p).

Quick Overview for PD-L1 antibody (Isoform 1) (ABIN6990335)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 60-110, Isoform 1

Specificity: PD-L1 antibody has no cross-reactivity to PD-L2.

Purification: PD-L1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PD-L1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human PD-L1 isoform 1. The immunogen is located within amino acids 60 - 110 of PD-L1.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, IHC-P: 2.5-5 μ,g/mL, IF: 20 μ,g/mL, Flow Cyt: 0.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human and rat samples, Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PD-L1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).

Molecular Weight: Predicted: 33 kDa

Observed: 37 kDa

Gene ID: 29126

NCBI Accession: NP_054862

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints