IRAK4 antibody (C-Term)

Catalog No. ABIN6990351

This anti-IRAK4 antibody is a Rabbit Polyclonal antibody detecting IRAK4 in WB, ELISA, ICC and IF. Suitable for Human.

Quick Overview for IRAK4 antibody (C-Term) (ABIN6990351)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IRAK4 Antibody

Binding Specificity: C-Term

Specificity: IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.

Purification: IRAK-4 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-IRAK4 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK4. The immunogen is located within the last 50 amino acids of IRAK4.

Isotype: IgG

Application Details

Application Notes: WB: 1-4 μ,g/mL, ICC: 10 μ,g/mL, IF: 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRAK-4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRAK-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK-4

Background: IRAK-4 Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κ,B and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-κ,B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

Molecular Weight: Predicted: 52kD

Observed: 55 kD kDa

Gene ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades