IRAK3 antibody (C-Term)

Catalog No. ABIN6990358

This Rabbit Polyclonal antibody specifically detects IRAK3 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IRAK3 antibody (C-Term) (ABIN6990358)

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRAK3 Antibody

Binding Specificity: C-Term

Specificity: IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.

Purification: IRAK-M Antibody is affinity chromatography purified via peptide column.

Immunogen: IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M. The immunogen is located within the last 50 amino acids of IRAK-M.

Isotype: IgG

Application Details

Application Notes: IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μ,g/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in mouse and rat samples, Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IRAK-M Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IRAK-M antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IRAK3

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Alternative Name: IRAK-M

Background: IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Molecular Weight: Predicted: 66 kDa

Observed: 70 kDa

Gene ID: 11213

NCBI Accession: NP_009130

UniProt: Q9Y616

Pathways: TLR Signaling, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades