MTBP antibody (AA 110-160)

Catalog No. ABIN6990370

Product Details anti-MTBP Antibody

Target: MTBP (MDM2 Binding Protein (MTBP))

Binding Specificity: AA 110-160

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: MTBP Antibody is affinity chromatography purified via peptide column.

Immunogen: MTBP antibody was raised against a 18 amino acid peptide near the center of human MTBP. The immunogen is located within amino acids 110 - 160 of MTBP.

Isotype: IgG

Application Details

Application Notes: MTBP antibody can be used for detection of MTBP by Western blot at 1 to 2 μ,g/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MTBP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MTBP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MTBP

Target: MTBP (MDM2 Binding Protein (MTBP))

Alternative Name: MTBP (MTBP Products)

Synonyms: MDM2BP antibody, AI429604 antibody, MDM2 binding protein antibody, Mdm2, transformed 3T3 cell double minute p53 binding protein antibody, MTBP antibody, Mtbp antibody

Background: MTBP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.

Molecular Weight: Predicted: 104 kDa

Observed: 104 kDa

Gene ID: 27085

NCBI Accession: NP_071328

UniProt: Q96DY7