ADAM17 antibody (C-Term)

Catalog No. ABIN6990406

This Rabbit Polyclonal antibody specifically detects ADAM17 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for ADAM17 antibody (C-Term) (ABIN6990406)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ADAM17 Antibody

Binding Specificity: C-Term

Specificity: 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.

Purification: TACE Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-TACE antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE.

Isotype: IgG

Application Details

Application Notes: WB: 0.25-2 μ,g/mL, ICC: 10 μ,g/mL, IF: 10 μ,g/mL.

Antibody validated: Western Blot in human and rat samples, Immunocytochemistry in human samples, and Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TACE Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TACE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: TACE

Background: TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α, is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α,. TNF-α, converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Molecular Weight: Predicted: 93kD

Observed: 93-125kD (Post-modification: 9 N-linked glycosylation) kDa

Gene ID: 6868

NCBI Accession: NP_003174

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus