CUL9 antibody (C-Term)

Catalog No. ABIN6990433

This Rabbit Polyclonal antibody specifically detects CUL9 in WB, ICC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CUL9 antibody (C-Term) (ABIN6990433)

Target: CUL9 (Cullin 9 (CUL9))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CUL9 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF)

Product Details anti-CUL9 Antibody

Binding Specificity: C-Term

Purification: PARC Antibody is affinity chromatography purified via peptide column.

Immunogen: PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC. The immunogen is located within the last 50 amino acids of PARC.

Isotype: IgG

Application Details

Application Notes: PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PARC Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PARC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CUL9

Target: CUL9 (Cullin 9 (CUL9))

Alternative Name: PARC

Background: PARC Antibody: The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.

Gene ID: 23113

NCBI Accession: NP_055904

UniProt: Q8IWT3