TRIM5 antibody (C-Term)

Catalog No. ABIN6990446

The Rabbit Polyclonal anti-TRIM5 antibody is suitable to detect TRIM5 in samples from Human. It has been validated for WB, ELISA, IHC (p) and IF.

Quick Overview for TRIM5 antibody (C-Term) (ABIN6990446)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-TRIM5 Antibody

Binding Specificity: C-Term

Purification: TRIM5 delta Antibody is affinity chromatography purified via peptide column.

Immunogen: TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta. The immunogen is located within the last 50 amino acids of TRIM5 delta.

Isotype: IgG

Application Details

Application Notes: TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μ,g/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM5 delta Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM5 delta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5 delta

Background: TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α, variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ, isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ, also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Molecular Weight: Predicted: 36 kDa

Observed: 33 kDa

Gene ID: 85363

NCBI Accession: NP_149084

UniProt: Q9C035

Pathways: Activation of Innate immune Response