ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Purification
DAD1 Antibody is affinity chromatography purified via peptide column.
Immunogen
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1.
DAD1
Reactivity: Human, Mouse, Rat
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μ,g/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
DAD1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Background
DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.