ECSIT antibody (C-Term)

Catalog No. ABIN6990485

The Rabbit Polyclonal anti-ECSIT antibody is suitable to detect ECSIT in samples from Human and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for ECSIT antibody (C-Term) (ABIN6990485)

Target: ECSIT

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECSIT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ECSIT Antibody

Binding Specificity: AA 290-340, C-Term

Purification: ECSIT Antibody is Ion exchange chromatography purified.

Immunogen: ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT. The immunogen is located within amino acids 290 - 340 of ECSIT.

Isotype: IgG

Application Details

Application Notes: ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ECSIT Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ECSIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ECSIT

Target: ECSIT

Alternative Name: ECSIT

Background: ECSIT Antibody: Activation of NF-κ,B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Gene ID: 51295

NCBI Accession: NP_057665

UniProt: Q9BQ95

Pathways: TLR Signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome